CRISPR Mouse Activation C2C12

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using hCas9 to perform CRISPR Rat - Activation CD2

Get tips on using MLM3636 to perform CRISPR Human - Activation VEGFA

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using Cas9m4-VP64 to perform CRISPR Human - Activation NEUROD1

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Activation α-synuclein

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation ASCL1

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation RhoGDIα