CRISPR Rat Deletion BMSCs

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Deletion ATDC5 Nfatc1

Get tips on using pX330A-1x3 to perform CRISPR Mouse - Deletion ATDC5 Perk

Get tips on using gRNA_Cloning Vector to perform CRISPR Mouse - Deletion L929 Gβ2

Get tips on using pSpCas9 (PX165) to perform CRISPR Mouse - Deletion C2C12 Klf5

Get tips on using pSpCas9 (PX165) to perform CRISPR Mouse - Deletion αT3 IP3R1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion STING exon 5

Get tips on using gRNA_Cloning Vector to perform CRISPR Mouse - Deletion RMA cells Trh4

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Deletion 3T3-L1 TEAD

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.