DNA gel extraction / PCR product purification

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Get tips on using GenLadder 100 bp + 1.5 kbp (ready-to-use), DNA marker to perform DNA Ladder 100 bp

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida albicans

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using QIAamp MinElute Media Kit to perform DNA isolation / purification Tissue - genital / cervical samples

Get tips on using QIAseq FX Single Cell DNA Library Kit (96) to perform Whole Genome Amplification Parasites

Get tips on using NucleoSpin® Soil to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Get tips on using QIAamp UCP Pathogen Mini Kit to perform DNA isolation / purification Tissue - bronchoalveolar lavage

Get tips on using REPLI-g Advanced DNA Single Cell Kit (96) to perform Whole Genome Amplification Mouse