reporter-gene-assay-luciferase-bhk-21-baby-hamster-kidney-cells

Get tips on using Oris™ Pro Cell Migration Assay to perform Wound healing assay cell type - human HUVEC

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MDA-MB-231

Get tips on using WST-1 Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - MG-63

Get tips on using CyQUANT® Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - Saos-2

Get tips on using MTS Cell Proliferation Colorimetric Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - Panc-1

Get tips on using CyQUANT® Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - DU-145

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat endothelial progenitor cells

Get tips on using Pierce™ LDH Cytotoxicity Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - HEK 293

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.