rna-isolation-purification-cells-primary-mouse-ventricles

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A253

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MDCK

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized DH82

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Vero

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized OVCAR3

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Jurkat

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.