DNA isolation / purification Tissue

Get tips on using DNeasy UltraClean Microbial Kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

Get tips on using DNeasy Plant Mini Kit (250) to perform DNA isolation / purification Plants - Fungus

Get tips on using DNeasy 96 Plant Kit (6) to perform DNA isolation / purification Plants - Leaves

Get tips on using DNeasy mericon Food Kit (50) to perform DNA isolation / purification Processed food

Get tips on using DNeasy PowerMax Soil Kit (10) to perform DNA isolation / purification Soil samples

Get tips on using DNeasy PowerWater Sterivex Kit (50) to perform DNA isolation / purification Water samples

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Rat_Liver