Get tips on using Human Adiponectin ELISA Kit (ab108786) to perform ELISA Human - Adiponectin
Get tips on using Human Adiponectin/Acrp30 DuoSet ELISA to perform ELISA Human - Adiponectin
Get tips on using Rn_Ywhaz_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - H9c2 14-3-3 f/Ywhaz
The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.
Get tips on using Human Melanocyte Media to perform Mammalian cell culture media HEM
Get tips on using Human vWF-A2 DuoSet ELISA to perform ELISA Human - VWF-A2
Get tips on using Human PDGF-BB ELISA Kit to perform ELISA Human - PDGF-BB
Get tips on using Human PDGF-BB DuoSet ELISA to perform ELISA Human - PDGF-BB
Get tips on using KIM-1 (human) ELISA kit to perform ELISA Human - KIM-1
Get tips on using Human IGF1 ELISA Kit (ab100545) to perform ELISA Human - IGF-I
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