siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using EasySep™ Direct Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using MojoSort™ Human B Cell (CD43-) Isolation Kit to perform Cell Isolation B cell

Get tips on using EasySep™ Human Naïve B Cell Enrichment Kit to perform Cell Isolation Naive B cell

Get tips on using MojoSort™ Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Get tips on using EasySep™ Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Get tips on using MACSprep™ HLA B Cell Isolation Kit, human to perform Cell Isolation HLA B Cell

Get tips on using MACSprep™ HLA T Cell Isolation Kit, human to perform Cell Isolation HLA T Cell