Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Gut
Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Bronchi
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Rat cell lines
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram positive Clostridium difficile
Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram negative Salmonella enterica
Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized SK-BR-3
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized ZR-75-1
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