siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized human pancreatic cancer

Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - HeLa cervical cancer cells

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - HeLa cervical cancer cells

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Ovarian

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using EasySep™ Direct Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using MojoSort™ Human B Cell (CD43-) Isolation Kit to perform Cell Isolation B cell

Get tips on using EasySep™ Human Naïve B Cell Enrichment Kit to perform Cell Isolation Naive B cell