cell-cycle-assay-human-hela

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - A2780

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - K562

Get tips on using Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit to perform Cell cycle assay human - OVCAR-5

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HeLa

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - U20S

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - U87

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - FaDu

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - SW480

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - U87MG