Get tips on using Human ICAM1 ELISA Kit (CD54) (ab100640) to perform ELISA Human - ICAM-1/CD54
Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.
Get tips on using ON-TARGETplus Human PAK1 (5058) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Caco-2 PAK1
Get tips on using ON-TARGETplus Human MCL1 (4170) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - M245 MCL-1
Get tips on using ON-TARGETplus Human FOXM1 (2305) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MCF-7 FOXM1
Get tips on using ON-TARGETplus Human ERCC4 (2072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS XPF/ERCC4
Get tips on using ON-TARGETplus Human MOAP1 (64112) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A2780 MOAP-1
Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A431 RCP/RAB11FIP1
Get tips on using ON-TARGETplus Human ABCG2 (9429) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BC-1 BCRP
Get tips on using ON-TARGETplus Human RRM2 (6241) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCBL-1 RRM2
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