RNA sequencing Human

Get tips on using Human Genome CGH Microarray Kit 244A to perform Microarray Comperative genomic hybridization - Human HepG2

Get tips on using Human Synoviocyte Growth Medium to perform Mammalian cell culture media HFLS-OA

Get tips on using Human Apoptosis Array G1 to perform Apoptosis assay cell type - PC-3

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using A2B5 Antibody, anti-human/mouse/rat, APC to perform Flow cytometry Anti-bodies Human - A2B5

Get tips on using IntestiCult™ Organoid Growth Medium (Human) to perform 3D Cell Culture Media Human gastric organoids

Get tips on using IntestiCult™ Organoid Growth Medium (Human) to perform 3D Cell Culture Media Human pancreatic organoids

Get tips on using Purified anti-human Ki-67 Antibody to perform Flow cytometry Anti-bodies Human - Ki-67

Get tips on using APC anti-human Ki-67 Antibody to perform Flow cytometry Anti-bodies Human - Ki-67

Get tips on using PE Mouse anti-Human B7-H4 to perform Flow cytometry Anti-bodies Human - B7-H4