shRNA gene silencing Human TF‐1

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Cellular assays Cell line authentication Ovarian cancer cell line PA-1

Cellular assays Cell line authentication Renal carcinoma cell line Caki-1

Get tips on using ON-TARGETplus Rat Snap23 (64630) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Snap23

Products Horizon Discovery Ltd. ON-TARGETplus Rat Snap23 (64630) siRNA - Set of 4

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Products Sigma-Aldrich β-Gal Reporter Gene Assay, chemiluminescent

Get tips on using β-Galactosidase Reporter Gene Staining Kit to perform Reporter gene assay β-galactosidase substrates - HeLa cervical cancer cells

Products Sigma-Aldrich β-Galactosidase Reporter Gene Staining Kit

Get tips on using β-Galactosidase Reporter Gene Staining Kit to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Products Sigma-Aldrich β-Galactosidase Reporter Gene Staining Kit

Get tips on using Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Products BioLegend Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Gene expression arrays - Rhesus monkey brain tissue Biotin

Products Thermo Fisher Scientific GeneChip® Human Genome U133 Plus 2.0 Array

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase BHK-21 baby hamster kidney cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates HeLa cervical cancer cells

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