rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized D-17

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MA-104

Get tips on using QIAzol Lysis Reagent to perform RNA isolation / purification Cells - immortalized CT26

Get tips on using Gibco™Neurobasal™ Medium to perform 3D Cell Culture Media Mouse embryonic neurospheres

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A-1207

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized U-138

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized SHG-44

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MDAH 2774

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized OVCAR-3