shRNA gene silencing Mouse P19

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Mouse TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Mouse - TGF-beta 1

Get tips on using Mouse SDF-1 α / CXCL12 α ELISA Kit to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using Mouse IL-1 Beta PicoKine™ ELISA Kit to perform ELISA Mouse - IL-1 beta

Get tips on using Mouse ICAM-1 / CD54 PicoKine™ ELISA Kit to perform ELISA Mouse - ICAM-1/CD54

Get tips on using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb to perform siRNA / miRNA gene silencing Human - COV-434 SAPK/JNK

Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2

Get tips on using Cyclin E1 (HE12) Mouse mAb #4129 to perform Western blotting Cyclin E

Get tips on using Human/Mouse Active Caspase-3 Antibody to perform Western blotting Caspase-3