siRNA / RNAi /miRNA transfection Human Cells KG-1

Get tips on using Notch 1 Antibody (A-8): sc-376403 to perform Immunohistochemistry Human - Notch1

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HK-2 cells

Get tips on using PM-1 Subcutaneous Preadipocyte Medium to perform Stem cell culture media hAdipose derived stem cells

Get tips on using Anti-Beclin 1 antibody (ab62557) to perform Autophagy assay cell type - Proximal tubular cells (rPT)

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / miRNA gene silencing Mouse - Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.