Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary human cardiac fibroblasts
Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue
Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood monocytes
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain
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