Get tips on using PureZOL™ RNA Isolation Reagent to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using illustra RNAspin Mini Kit to perform RNA isolation / purification Cells - immortalized BRL 3A
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized DG-75
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized THP-1
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized PC-3
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized NTERA-2
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized NK-92MI
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