CRISPR Rat Activation

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using SASI_Hs01_00024301 to perform siRNA / miRNA gene silencing Human - MOLT4 RAG1

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Raji

Get tips on using LC3B Antibody to perform Autophagy assay cell type - RAW 264.7

Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Raji

Get tips on using TruSeq Stranded Total RNA to perform RNA sequencing Mouse - RAW264.7

Get tips on using ATG12 Antibody (166) to perform Autophagy assay cell type - RAW 264.7

Get tips on using Cytokeratin 19 Antibody to perform Immunohistochemistry Cytokeratin 19 - Rabbit Mouse -NA-