crispr-mouse-activation-3t3-l1-c-ebp

Get tips on using Stk11 siRNA to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 Stk11

Get tips on using pLKO5.sgRNA.EFS.GFP to perform CRISPR Mouse - Activation Neuro-2a Smn1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using ANT2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 ANT2

Get tips on using Atf4 siRNA(m) to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 ATF4

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - 3T3-L1

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Mouse - Cytochrome c

Get tips on using Mouse C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Mouse - C-Reactive Protein/CRP

Get tips on using APC anti-mouse CD117 (c-kit) Antibody to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit