Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay Hep G2
Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay HEK 293T
Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hESC-EC
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MIA PaCa-2
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MDA-MB-231
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment