Stem cell Differentiation media

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Bacillus cellulosilyticus

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HUVEC

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HeLa

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized HT-29

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized BxPC-3

Get tips on using RNeasy Protect Cell Mini Kit to perform RNA isolation / purification Cells - immortalized C6/36

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Human CD14+ cells

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Rat_Mesenteric fat

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse skeletal muscle cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.