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Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Tissue - Rat Ovaries

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis

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