siRNA / miRNA gene silencing Human Aortic smooth muscle cell

Get tips on using Oligofectamine™ Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - A-10 Cationic lipid based

Get tips on using Xfect™ Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - H9c2 Cationic and neutral lipids

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / RNAi /miRNA transfection Mouse - Glomerular Mesangial cells polymer / lipid

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - RAW 264.7

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - SH-SY5Y

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - MIA PaCa-2

Get tips on using β-Galactosidase Reporter Gene Staining Kit to perform Reporter gene assay β-galactosidase substrates - mouse embryo tissue

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Gene expression arrays - Rhesus monkey brain tissue Biotin

Get tips on using SuperLight™ Dual Luciferase Reporter Gene Assay Kit to perform Reporter gene assay luciferase - HepG2 and Huh7 cells

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.