Site Directed Mutagenesis (SDM) Human Point mutation MDA-MB-231

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using MagniSort™ Human pan-Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using B Cell Isolation Kit II, human to perform Cell Isolation B cell

Get tips on using Human/Mouse Active Caspase-3 Antibody to perform Western blotting Caspase-3

Get tips on using ScriptSeq Complete Kit (Human/Mouse/Rat) to perform RNA sequencing Mouse - J774

Get tips on using Quantifiler™ Human DNA Quantification Kit to perform DNA quantification Brain tissue

Get tips on using PE Mouse Anti-Human CD61 Clone VI-PL2 to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using PE Mouse Anti-Human CD26 Clone M-A261 to perform Flow cytometry Anti-bodies Human - CD26

Get tips on using PE-Cy™7 Mouse Anti-Human CD10 to perform Flow cytometry Anti-bodies Human - CD10

Get tips on using PE-Cy™7 Mouse Anti-Human CD56 to perform Flow cytometry Anti-bodies Human - CD56