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Found 3 matching solutions for this experiment
| Prepare a staining solution of 2 uM calcein AM/4 uM EthD-III by adding 5 uL of 4 mM calcein AM and 20 uL of 2 mM EthD-III to 10 mL of PBS or other serum-free buffer or medium
|Incubate the cells for 30-45 minutes at room temperature.
|Mix 1 µL of Solution A and 1 µL of Solution B in 1 mL of Staining Buffer to make a staining solution
|Incubate for 15 minutes at 37°C.
|Mix 2 µM Calcein AM and 4 µM Ethidium homodimer‐1 in PBS and incubate at 37°C for 20 min.
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