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Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates mouse cardiac fibroblasts

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates rat nucleus pulposus

Get tips on using 8 µm Chemotaxis Assays, 96-Well Format to perform Cell migration / Invasion cell type - MCF-10A

Products Cell Biolabs 8 µm Chemotaxis Assays, 96-Well Format

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - PANC-1

Products Cell Biolabs 8 µm Chemotaxis Assays, 24-Well Format

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - RAW 264.7

Products Sigma-Aldrich β-Gal Reporter Gene Assay, chemiluminescent

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - SH-SY5Y

Products Sigma-Aldrich β-Gal Reporter Gene Assay, chemiluminescent

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - MIA PaCa-2

Products Sigma-Aldrich β-Gal Reporter Gene Assay, chemiluminescent

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - 6B5N

Products Bio-Rad Laboratories DC™ Protein Assay Kit I

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - A7r5

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - A7r5

Products Bio-Rad Laboratories DC™ Protein Assay Kit I

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