rna-isolation-purification-cells-primary-canine-peripheral-blood-mononuclear-cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using RNA-Bee to perform RNA isolation / purification Tissue - Mouse Colon

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Retina

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Cerebellum

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Brainstem

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Brain

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat hepatic stellate cells

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat hepatic stellate cells

Get tips on using Dynabeads™ mRNA Purification Kit to perform RNA isolation / purification Tissue - Rat Adrenal glands