DNA isolation / purification Cells Primary cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDA-MB-361

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDA-MB-157

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized OVCAR-3

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A-1207

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized U-138

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized SHG-44

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MDAH 2774

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized OVCAR-3