siRNA / miRNA gene silencing Human HT-29

Get tips on using Renilla luciferase vector, pGL4.74 to perform Reporter gene assay luciferase - primary human endometrial stromal cells

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using Human Melanocyte Media to perform Mammalian cell culture media HEM

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Human vWF-A2 DuoSet ELISA to perform ELISA Human - VWF-A2

Get tips on using Human PDGF-BB ELISA Kit to perform ELISA Human - PDGF-BB

Get tips on using Human PDGF-BB DuoSet ELISA to perform ELISA Human - PDGF-BB

Get tips on using KIM-1 (human) ELISA kit to perform ELISA Human - KIM-1

Get tips on using Human IGF1 ELISA Kit (ab100545) to perform ELISA Human - IGF-I

Get tips on using HO-1 (human), ELISA kit to perform ELISA Human - HO-1