Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using Secrete-Pair™ Dual Luminescence Assay Kit to perform Reporter gene assay β-galactosidase substrates - HUVEC
Get tips on using AllPrep DNA/RNA Mini Kit to perform AAA for reviews
Get tips on using LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells to perform Live / Dead assay mammalian cells - K562
Get tips on using TriDye™ Ultra Low Range DNA Ladder to perform DNA Ladder Low Range
Get tips on using E-Gel™ 50 bp DNA Ladder to perform DNA Ladder 50 bp
Get tips on using 100 bp DNA Ladder Ready to Load to perform DNA Ladder 100 bp
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