Get tips on using EpiTect Fast DNA Bisulfite Kit (200) to perform Bisulfite DNA Modification Tissue
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Get tips on using QIAxcel DNA Fast Analysis Kit (3000) to perform Analysis of DNA fragments
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The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
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