Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Vibrio cholerae
Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Escherichia coli
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse heart
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse aorta
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse liver tissue
Get tips on using EasySep™ Direct Human T Cell Isolation Kit to perform Cell Isolation Human T cells
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rat skin tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rat prostate tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Lamb muscle tissue
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