Microarray Human Precision cut lung slices

Get tips on using QIAzol Lysis Reagent to perform RNA isolation / purification Tissue - rat lung tissue

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat lung tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - mouse lung tissue

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Lung

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - Mouse Lung

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Tissue - Mouse lung tissue

Get tips on using PureLink Genomic DNA Mini Kit to perform DNA isolation / purification Tissue - lung

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - lung

Get tips on using Gibco™ IMDM, GlutaMAX™ Supplement to perform 3D Cell Culture Media hiPSC-derived lung organoids

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).