Protein expression and purification

Get tips on using MinElute 96 UF PCR Purification Kit (24) to perform DNA isolation / purification Plasmid purification

Get tips on using QIAquick PCR Purification Kit to perform Plasmid Isolation Klebsiella pneumoniae

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Tissue - placenta

Get tips on using MasterPure™ Yeast RNA Purification Kit to perform RNA isolation / purification Yeast - Candida albicans

Get tips on using RiboPure™ RNA Purification Kit, yeast to perform RNA isolation / purification Yeast - Candida albicans

Get tips on using RiboPure™ RNA Purification Kit, yeast to perform RNA isolation / purification Yeast - Ashbya gossypii

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Yeast - Candida parapsilosis

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Yeast - Pichia pastoris

Get tips on using RiboPure™ RNA Purification Kit, yeast to perform RNA isolation / purification Yeast - Saccharomyces cerevisiae

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.