Western blot 1,4 β-N-acetyl-D-glucosamine Triticum vulgaris

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

Get tips on using ON-TARGETplus Mouse Hspa5 (14828) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MC3T3-E1 Grp78/Hspa5

Get tips on using Streptavidin-R-PE to perform Protein tag Detection of biotinylated proteins

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse dorsal skin Biotin

Get tips on using mericon DNA Bacteria Plus Kit (50) to perform DNA isolation / purification Bacteria - Gram positive Clostridium botulinum

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

Get tips on using Luria Bertani Broth, Miller (Miller Luria Bertani Broth) to perform Bacterial cell culture media Escherichia coli

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MARC-145

Get tips on using Goat Anti-Type III Collagen-BIOT to perform Immunohistochemistry Collagen Type III - Goat Mouse Biotin

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Mouse skin tissue Biotin