shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD4 to perform Flow cytometry Anti-bodies Mouse - CD4

Get tips on using PolyFect Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Chang Liver cells

Get tips on using Stealth siRNA_SPI1 to perform siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - SKOV-3 Wee-1

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RBM3

Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RIP3

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1

Get tips on using siRNA FOXM1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 FOXM1

Get tips on using HuR siRNA to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR Lipid