siRNA / miRNA gene silencing Human LAD2

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT

Get tips on using β-Galactosidase Staining Kit to perform Reporter gene assay β-galactosidase substrates - human knee bone tissue

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Whole blood (human) FKBP5

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Get tips on using p62 (human) polyclonal antibody to perform Immunohistochemistry Mouse - p62

Get tips on using Human MMP-1 Antibody to perform Western blotting MMP1

Get tips on using Human GDNF DuoSet ELISA to perform ELISA Rat - GDNF

Get tips on using Human VEGF PicoKine™ ELISA Kit to perform ELISA Human - VEGF

Get tips on using Human Sonic Hedgehog ELISA Kit (ab100639) to perform ELISA Human - ShhN