siRNA / miRNA gene silencing Rat Retinal stem cells

Get tips on using Alexa Fluor® 488 Rat Anti-Mouse CD146 to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using PE-Cy™7 Rat anti-Mouse CD117 to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using PE-Cy™7 Rat Anti-Mouse CD31 to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using miRNA Complete Labeling and Hyb Kit to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Cyanine 3-pCp

Get tips on using Rat GFR alpha-1/GDNF R alpha-1 Antibody to perform Immunohistochemistry Mouse - GFRA1

Get tips on using Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO) to perform Immunohistochemistry Mouse - CD24

Get tips on using Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO) to perform Immunohistochemistry Mouse - CD24

Get tips on using Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody to perform Western blotting AKT