siRNA / miRNA gene silencing Human HeLa EPAS-1

Get tips on using TurboFect Transfection Reagents to perform siRNA / RNAi /miRNA transfection Rat - H9c2 Cationic and neutral lipids

Get tips on using TransIT-TKO Transfection Reagent to perform siRNA / RNAi /miRNA transfection Mouse - Primary Splenocytes Polymer / lipid

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - AR42J Lipid based

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - IEC-6 Lipofectamine

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - HeLa

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Autophagy assay cell type - HeLa