Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Kidney
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Heart
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Eye
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Colon
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Decidua
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Bladder
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Adipose
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Gibco™Neurobasal™ Medium to perform Stem cell culture media NCH421K cells primary glioma
Get tips on using Gentra Puregene Mouse Tail Kit (4 g) to perform DNA isolation / purification Tissue - murine tail biopsies
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