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Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates U87 and U251 glioblastoma cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates human MSCs (mesenchymal stem cells)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates rat mesenchymal stem cells (MSCs)

Get tips on using IMAGEN™ Herpes Simplex Virus (HSV) Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus

Products Thermo Fisher Scientific IMAGEN™ Herpes Simplex Virus (HSV) Kit using Direct Immunofluorescence Assay

Get tips on using IMAGEN™ Respiratory Syncytial Virus Kit (RSV) using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus

Products Thermo Fisher Scientific IMAGEN™ Respiratory Syncytial Virus Kit (RSV) using Direct Immunofluorescence Assay

Get tips on using SPLInsert™ Hanging to perform Cell migration / Invasion cell type - HaCat

Products SPL Life Sciences SPLInsert™ Hanging

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - MDA-MB-231

Products Thermo Fisher Scientific Pierce™ Coomassie (Bradford) Protein Assay Kit

Get tips on using OxiSelect™ Comet Assay Kit (3-Well Slides) to perform DNA Damage Assay HeLa

Products Cell Biolabs OxiSelect™ Comet Assay Kit (3-Well Slides)

Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - human MSCs (mesenchymal stem cells)

Products Promega β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Get tips on using Qubit® RNA HS Assay Kit to perform RNA quantification Fuorimetric

Products Thermo Fisher Scientific Qubit® RNA HS Assay Kit

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