Cell Isolation

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MDA-MB-231

Get tips on using 96-Well Cell Invasion Assay, Basement Membrane to perform Cell migration / Invasion cell type - SK-GT-4

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - FaDu human squamous cell carcinoma

Get tips on using Cell Proliferation Kit I (MTT) to perform

Get tips on using Oris™ Pro Cell Migration Assay to perform Wound healing assay cell type - human MDA-MB-231

Get tips on using ToxCount™ Cell Viability Assay to perform Live / Dead assay mammalian cells - glioblastoma stem cells

Get tips on using MEGMTM Mammary Epithelial Cell Growth Medium BulletKitTM to perform 3D Cell Culture Media BT-549 cells-Mammospheres

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse skeletal muscle cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer