ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α -NA- Human

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using MethylCap kit to perform DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using pFastBac1-sccaIL12opt to perform Protein Expression Eukaryotic cells - Hi5 IL12 canine

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit to perform Angiogenesis assay human - PMVEC

Get tips on using Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit to perform Angiogenesis assay human - hRMVEC

Get tips on using Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit to perform Angiogenesis assay human - HUVEC

Get tips on using SimpleChIP® Plus Sonication Chromatin IP Kit #56383 to perform ChIP Human - SW480