PCR Quantitative real-time PCR

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat astrocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary canine osteoblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized PC-3

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary canine skin fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat striatal neurons

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat lung fibroblasts