Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U266 -
Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.
Get tips on using Corning® Transwell® polycarbonate membrane cell culture inserts to perform Cell migration / Invasion cell type - RAW 264.7
Get tips on using Oris™ Universal Cell Migration Assembly Kit, 96 wells to perform Cell migration / Invasion cell type - MCF-10A
Get tips on using Cultrex 3D Culture BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - BxPC-3
Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 5 µm to perform Cell migration / Invasion cell type - SaOS-2
Get tips on using Corning® Transwell® polycarbonate membrane cell culture inserts to perform Cell migration / Invasion cell type - MHCC-97H
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - A549
Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 3 µm to perform Cell migration / Invasion cell type - isolated human neutrophils
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