CRISPR Rat Deletion INS-1 832/13

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized KG-1

Get tips on using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - THP-1

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - PA-1

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - OECM-1

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - THP-1

Get tips on using BH ladder :: GD OneMARK B RTU Ladder to perform DNA Ladder 1 kb

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)