Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Spleen
Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Retina
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Liver
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Intestine
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Heart
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Gut
Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Bronchi
Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat
Get tips on using PureLink ™ miRNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis
Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
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