Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - SKOV-3
Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - OVCAR-3
Get tips on using BrdU Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - OVCAR-3
Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - SH-SY5Y
Get tips on using Cell Proliferation Reagent WST-1 to perform Cell cytotoxicity / Proliferation assay cell type - L-02
Get tips on using MTT Cell Viability Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - 3T3-L1
Get tips on using Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit to perform Angiogenesis assay human - HMVEC
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
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